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Journal: Cell reports

Article Title: Patient-specific iPSCs carrying an SFTPC mutation reveal the intrinsic alveolar epithelial dysfunction at the inception of interstitial lung disease

doi: 10.1016/j.celrep.2021.109636

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Approximately 5×10 6 cells were centrifuged at 200 g for 5 minutes, resuspended in a mixture containing P3 solution and supplement (P3 Primary Cell 4D-Nucleofector X Kit L, Lonza) as well as 5 μg CRISPR/Cas9 plasmid and 5 μg oligo donor, and nucleofected using program code CB-150 in the 4D Nucleofector X System (Lonza).

Techniques: Recombinant, Electron Microscopy, Lysis, Reverse Transcription, Flow Cytometry, Luminex, Expressing, Variant Assay, Sequencing, Software, Gentle, Saline, Modification